Juventus, who have won four successive Serie A titles, are playing Napoli on Saturday at the San Siro.
They are the first team to play in this tie, having lost 4-1 at Sampdoria on Saturday.
The win sees Juve reach the last eight of the competition, a feat which only two other teams have achieved in their history.
The two sides will meet for the first time since September last year.
“Napoli, who came to the San Mames with the same attitude we did, showed they were a different team and we knew we would win,” Juve coach Maurizio Sarri said.
“I was quite proud when I saw that they were not afraid of us.
But we still have a few things to work on.”
Napoli won the last meeting, 3-1, in a shootout in January.
Sarri’s side had been without defender Antonio Candreva since he underwent surgery for a suspected bacterial infection in December.
Juve’s defensive line was made up of Alessandro Nesta, Simone Zaza, Alessandro Romagnoli, Marco Parolo, Daniele De Rossi and Alessandro Cifuoco, who had been injured in the previous two matches.
With two games remaining, the Giallorossi will try to get a win over their rivals, who are in Group G. Sarri said the team had “lost the battle of the hearts” on Saturday, with their midfield and defence lacking a “high level of concentration”.
“We know how tough it is to play against Juventus and how we have to keep fighting,” he said.
He added: “I think we have played well and showed how important it is for us to win.”
On Sunday, Juventus play Udinese at the Stadio Olimpico in Turin.
Udinese, who lost 2-1 to Napoli in the Champions League on Saturday night, play at home against Udinese on Tuesday.